Thursday, February 8, 2018

Thank You Science! Using Nanotechnology to test for Oak Wilt

Exciting news from the Tree World I inhabit. New technology developed by the University of Minnesota College of Food, Agricultural and Natural Resource Sciences (CFANS) offers a simple
and affordable diagnostic test  for Oak Wilt utilizing nanotechnology and gold.

Pictured below is a microfluidic device used to isolate the Oak Wilt fungus from wood chip samples.
Picture credit-University of Minnesota

Oak Wilt is a devastating vascular fungus that quickly kills Oak trees. Arborists in the Kansas
City Metro area deal with Oak Wilt every year. The disease is not particularly widespread in our area,
but it can move quickly through root grafting in wooded areas with multiple Oak trees.
This can be a painful and expensive experience for homeowners with wooded lots.

Past testing for Oak Wilt has been a slow process, sometimes waiting for weeks to receive a positive diagnosis-it is often difficult to get a positive diagnosis from wood samples, creating a slow and frustrating process for everyone involved.

This latest scientific breakthrough enables Oak wilt detection within 30 minutes of sampling at a fraction of the cost (less than $5). This new technology uses gold nanoparticles to generate a chemiluminescent signal in the presence of Oak Wilt-this chemical signal must be read by a specialized hand held reader.

The team working on this project are refining a portable system for use in the field,
eliminating the need to send samples to the lab for confirmation. The field project requires only dipping wood chips from infected trees into a solution to extract DNA, then mixing with a second solution of gold nanoparticles to allow detection using a hand held luminometer.

The innovation by Abbas' research lab enables oak wilt detection within 30 minutes of sampling and at a fraction of the cost: less than five dollars per sample. The technology uses the agglomeration of gold nanoparticles to generate a chemiluminescent signal that can be read by a hand-held reader, in the presence of the oak wilt fungus DNA. The team is now refining a portable system that allows early detection of the disease in the field without sending samples to the laboratory. The requires only dipping wood chips obtained from infected trees into a first solution to extract the DNA, then mixed with a second solution of to allow detection using a hand-held luminometer.

Read more at: https://phys.org/news/2017-11-technology-oak-wilt-faster.html#jCp
The innovation by Abbas' research lab enables oak wilt detection within 30 minutes of sampling and at a fraction of the cost: less than five dollars per sample. The technology uses the agglomeration of gold nanoparticles to generate a chemiluminescent signal that can be read by a hand-held reader, in the presence of the oak wilt fungus DNA. The team is now refining a portable system that allows early detection of the disease in the field without sending samples to the laboratory. The requires only dipping wood chips obtained from infected trees into a first solution to extract the DNA, then mixed with a second solution of to allow detection using a hand-held luminometer.

Read more at: https://phys.org/news/2017-11-technology-oak-wilt-faster.html#jCp
Thank you science! This is going to speed up the process of  testing for Oak Wilt, help Arborists
and homeowners and should ultimately save thousands of Oak trees that would be lost to the rapid spread of Oak Wilt.


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